Capillary Gel Electrophoresis
نویسنده
چکیده
منابع مشابه
Capillary electrophoresis is essential for microsatellite marker based detection and quantification of adulteration of Basmati rice (Oryza sativa).
Microsatellite markers are employed for genotyping of Basmati varieties and assaying purity of market samples. However, employment of diverse electrophoresis techniques across laboratories has resulted in inconsistent allele sizes, creating doubts about the suitability of the assay. This study evaluated agarose gel electrophoresis, slab gel electrophoresis, and capillary electrophoresis techniq...
متن کاملQuantitative analysis of serum proteins separated by capillary electrophoresis.
The possibility of open tubular capillary electrophoresis for clinical diagnostic use is examined. Capillary electrophoresis was performed in an untreated 50 microns (i.d.) x 100 cm (65 cm to detector) capillary with detection of absorbance at 200 nm. Conditions for the separation of serum proteins without adsorption to the capillary surface were established. Quantitative analyses of serum samp...
متن کاملPurity and Content Analysis of Oligonucleotides by Capillary Gel Electrophoresis
Chromatography and electrophoresis are the most common techniques available for the separation of mixtures. For the analysis of large molecules, capillary gel electrophoresis (CGE) was not introduced until the late 1980s, well after most chromatographic techniques. Guttman et al.,1 Cohen et al.,2 and Kasper et al.,3 all independently, combined the principles of slab gel electrophoresis with the...
متن کاملInterpretation and clinical significance of small monoclonal peaks in capillary electrophoresis.
BACKGROUND Although the testing mechanism and interpretation criteria for capillary electrophoresis differ from those for gel-based electrophoresis, there are not that many reports on the efficacy of capillary electrophoresis. MATERIALS AND METHODS We performed a retrospective analysis, using the Laboratory Information System (LIS) to review a total of 163 capillary electrophoresis results fr...
متن کاملUse of capillary electrophoresis as a method development tool for classical gel electrophoresis.
Capillary electrophoresis (CE) was used to optimize the buffer pH, ionic strength and sulfated cyclodextrin concentrations for enantiomeric separation of piperoxan. These enantioseparation conditions were then applied to a classical gel electrophoresis system. Binding constants of the sulfated beta-cyclodextrin-piperoxan couple were approximated using CE and the effects of organic solvents on t...
متن کاملDetection and classification of paraproteins by capillary immunofixation/subtraction.
A selection of 58 specimens with a monoclonal component identified by immunoelectrophoresis and/or immunofixation was analyzed with the immunosubtraction procedure on the Paragon 2000 capillary electrophoresis system. The capillary system detected 93% of the paraproteins and, using immunosubtraction, correctly identified 91% of the paraproteins. Paraproteins that were detected by immunofixation...
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